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Spinal Nerve Ligation (SNL)

A preclinical peripheral nerve injury model for neuropathic pain. 


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Preclinical Neuropathic Pain Model


In the SNL model of peripheral neuropathy, the L5 and L6 spinal nerves are isolated and can either be cut or tightly ligated with surgical silk. Axonal degeneration occurs, with all types of axons being approximately equally affected. Pronounced mechanical allodynia follows, accompanied by spontaneous pain behaviors, cold allodynia, which lasts for months with no recovery. 

Study Options

  • Rat L5/L6 ligation
  • Rat L5 only ligation
  • Mouse L5 ligation
  • Model length: up to 30 days
  • Positive controls: Morphine, Gabapentin, Pregabalin
  • Readouts: tactile allodynia (von Frey)
  • Endpoints: histology, biomarker analysis (protein or mRNA levels in sciatic nerve or serum/plasma)
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Von Frey

Effects of morphine on Von Frey in the SNL model of peripheral neuropathy.

Von Frey_SNL model_MD Biosciences

Paw Pressure

Changes in paw pressure (g) following Spinal Nerve Ligation in the SNL peripheral neuropathy model.

Paw Pressure_SNL Model_MD Biosciences


If you are ready to discuss how a partnership can fit into your development program, our scientists are eager to explore the possibilities with you. Like many other pharmaceutical and medical device developers, you can rely on predictive preclinical data.

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