Biomarker Analysis

Biomarker Assays

• Multiplex Analysis

  Multiplex analysis allows the detection of up to 50 biomarkers in a single reaction with a small sample size. This provides the ability to measure biomarker changes as a result of disease progression or therapeutic intervention in limited samples available in preclinical studies.

   

• Flow Cytometry

  Flow cytometry can be used to characterize cell populations and detect biomarkers on individual cells, which allows a greater understanding into the cellular changes that can often be identified before morphological changes are visible.

  

   

• Immunohistochemistry

  Histopathological evaluations form a highly valuable component of preclinical, translational and clinical studies alike, and add spatial, contextual and temporal information directly from tissues of interest.

  • Biomarkers in skin, sciatic nerve, spinal cord and other tissue samples
  • DRG Axonal staining
  • Intraepidermal nerve fiber (IENF) analysis in skin biopsy samples

   

  

   

• Electrophysiology

  Neurons are the fundamental units of the brain and nervous system, and are responsible for carrying information throughout the body using chemical and electrical signals (action potentials). The action potential causes the release of neurotransmitter into the synapse. Measuring action potentials by in vivo electrophysiology allows the study of functional properties of neurons and nerve conductivity. This can reveal pain-related electrical signals from the peripheral nerves to the brain. Electrophysiology enhances the understanding of neurological disorders as well as measures nerve regeneration, degeneration, and protection in response to treatments. In preclinical studies, electrophysiology can be a useful biomarker for measuring the function of the spinal motor neurons and sensory neurons, the functionality of peripheral motor nerves as well as spontaneous nerve firing.

  Read more about Electrophysiology capabilities

• DAMP and PAMP Assays

  • Tissue samples (from diseased and naive amimals) for biomarker research
  • Blood samples (from diseased and naive animals) for biomarker research

• Laser Doppler Blood Flow Imaging

  Used to measure cerebral blood flow of the cortex.

• Behavior Analysis

  Measure cognitive and motor defects, anxiety, stress, and depression. Learn more about behavior analysis. 

• ELISA

  Examples of common biomarkers measured with ELISA:

  • Neuro-specific enolase NSE
  • Protein S-100B
  • Ubiquitin C terminal hydrolase 1 (UCHL1)
  • Neurofilament light chain NfL
  • Tau protein
  • Glial fibrillary acidic protein GFAP