A novel preclinical animal model of complex regional pain syndrome-type I (CRPS-1)
Complex regional pain syndrome (CRPS) is a chronic pain condition often involving
hyperalgesia and allodynia of the extremities. CRPS is divided into CRPS-I and CRPS-II.
Type I occurs when there is no confirmed nerve injury. Type II is when there is known
associated nerve injury. Neuropathic inflammation, specifically activation of peripheral
nociceptors of C-fibers, has been shown to play a critical role in developing CRPS.
One of the models for CRPS is Chronic Post-Ischemia Pain Model (Hu et al., JOVE 155 (e60562): 1-6; 2020.
Neuropathic pain-like syndrome is induced in rats by prolonged hindpaw ischemia and reperfusion.
- Hindlimb ischemia using an o-ring
- 95-98% reduction in blood flow during ischemia period
- Model length: up to 14 days
- Positive controls: Morphine
- Readouts: tactile allodynia (Von Frey), paw thickness
Effects of morphine on Von Frey in the CPIP model.
The effect of morphine on paw thickness in the CPIP model.