MOG-Induced EAE

MOG-induced EAE Efficacy Model for MS Research

Experimental autoimmune encephalomyelitis (EAE) is primarily used as a non-clinical model of autoimmune inflammatory diseases of the CNS, and resembles many aspects of human Multiple Sclerosis (MS). The myelin oligodendrocyte glycoprotein (MOG)-induced EAE model is ideal for exploring this immune-mediated mechanism of neuroinflammation and demyelination. Our MOG-induced EAE model is well-characterized and commonly used in the development of a variety of MS therapeutics. MDB also provides OECD GLP-certified clinical batch release testing services for Copaxone® generics (Glatiramer Acetate). 

 

At MD Biosciences, the Multiple Sclerosis efficacy model contains immunization with Myelin Oligodendrocyte Glycoprotein (MOG35-55), which induces a chronic form of EAE in mice.

 

Download MOG-EAE poster below:

 

Characteristics of the Model Include: 

• Mononuclear inflammatory infiltration (macrophages, CD4+ T cells)
• Demyelination in the peripheral white matter of the spinal cord, which leads to gradual loss of motor function
• Meningitis and perivascular inflammatory cuffing in the cerebellum and hindbrain white matter
• This model has a reduced tendency to resolve inflammation and demyelination after the peak of disease, making it a good model of chronic inflammatory demyelination. 
  
  

Model Assessments Include:

• Clinical Score
• Electrophysiology recording and analysis 
• Biomarkers
• Histology for cellular infiltration and white matter damage (see below)
• Immunohistochemistry (IHC) for demyelination (see below)
• PK
• Neurometer CPT®: Evaluation of sensory nerve fibers function
• DANTEC Keypoint Focus tool for assessment 

H&E Staining

H&E staining can demonstrate inflammatory infiltrate into the spinal cord.

 Longitudinal Sections of Spinal Cord of MOG-Induced EAE Mice (H&E Staining):

Figure B. Low magnification showing diffuse histologic abnormalities. Figure C. Medium magnification showing multifocal vacuolation, predominantly in the ventral white matter. In some vacuoles there is myelin debris (black arrows). There is increased cellularity due to gliosis and inflammatory infiltration in the gray matter in the area surrounding the asterisk. Figure D. Medium magnification showing marked inflammatory infiltration of the terminal spinal cord (N = spinal nerves of the cauda equina). Figure E. High magnification showing diffuse mononuclear infiltration and multiple vacuoles containing myelin debris (black arrows).     

 

IHC Using Myelin Basic Protein (MBP) Antibody

IHC staining can demonstrate demyelinization through decreased signal for MBP

Longitudinal Sections of Spinal Cord of MOG-induced EAE Model in Mice (IHC with Anti-MBP Antibody)

Figure A. Normal region including white matter (mostly presented as longitudinal fibers) in both sides of the grey matter. Figure B. White matter damage including myelin debris (black arrows). Figure C. High magnification of digestion chambers (black arrows)

 

Other MS-EAE Models:

PLP

MBP