MOG-induced EAE Efficacy Model for MS Research
Experimental autoimmune encephalomyelitis (EAE) is primarily used as a non-clinical model of autoimmune inflammatory diseases of the CNS, and resembles many aspects of human Multiple Sclerosis (MS). The myelin oligodendrocyte glycoprotein (MOG)-induced EAE model is ideal for exploring this immune-mediated mechanism of neuroinflammation and demyelination. Our MOG-induced EAE model is well-characterized and commonly used in the development of a variety of MS therapeutics. MDB also provides OECD GLP-certified clinical batch release testing services for Copaxone® generics (Glatiramer Acetate).
At MD Biosciences, the Multiple Sclerosis efficacy model contains immunization with Myelin Oligodendrocyte Glycoprotein (MOG35-55), which induces a chronic form of EAE in mice.
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Characteristics of the MOG-EAE model
- Mononuclear inflammatory infiltration (macrophages, CD4+ T cells)
- Demyelination in the peripheral white matter of the spinal cord, which leads to gradual loss of motor function
- Meningitis and perivascular inflammatory cuffing in the cerebellum and hindbrain white matter
- This model has a reduced tendency to resolve inflammation and demyelination after the peak of disease, making it a good model of chronic inflammatory demyelination.
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Assessments
- Clinical Score
- Electrophysiology recording and analysis
- Biomarkers
- Histology for cellular infiltration and white matter damage (see below)
- Immunohistochemistry (IHC) for demyelination (see below)
- PK
Assessments
Inflammatory biomarkers provide information related to mechanisms contributing to inflammatory pain and how well compounds modulate the inflammatory and/or pain response.
![Pain behavioral anaysis](https://www.mdbneuro.com/hs-fs/hubfs/Icons/Pain.png?width=60&height=60&name=Pain.png)
Behavior
Pain behavior tests assess the response to various pain stimuli.
![biomarker](https://www.mdbneuro.com/hs-fs/hubfs/Icons/biomarker.png?width=60&height=60&name=biomarker.png)
Biomarkers
Evaluate pro-inflammatory and anti-inflammatory biomarkers from samples.
![histology](https://www.mdbneuro.com/hs-fs/hubfs/Icons/histology.png?width=60&height=60&name=histology.png)
Histology
IHC and histological staining to evaluate inflammatory markers in tissue samples.
![electrophysiology](https://www.mdbneuro.com/hs-fs/hubfs/Icons/electrophysiology.png?width=60&height=60&name=electrophysiology.png)
Electrophysiology
Evaluate spontaneous nerve firing using in vivo electrophysiology.
Scientific Data
Cytokines by multiplex analysis. Two markers from a cytokine profile in MOG-induced animals that were treated with vehicle or positive control. Day 15 was compared to day 26.
![Biomarkers - MOG EAE - MDB](https://www.mdbneuro.com/hs-fs/hubfs/2023%20MOG%20EAE/Biomarkers%20-%20MOG%20EAE%20-%20MDB.png?width=800&height=500&name=Biomarkers%20-%20MOG%20EAE%20-%20MDB.png)
Review the complete dataset.
EAE Pain Datasheet
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