Multiple Sclerosis Model
When conducting MS research, administration of one dose of MPB in a Lewis rat leads to a single relapse that is characterized by hind paw paralysis. This can be characterized histologically, by infiltration of mononuclear cells to the cervical spinal cord and formation of foci. This model is self-limiting and does not show demyelination.
The MBP-induced EAE efficacy model uses Lewis rats that are injected on day 0. The disease develops between days 10-13, and recovery occurs between day 18-21.
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Model Assessments Include:
Brain tissue - H&E Staining
H&E staining of brain tissue for detection of inflammatory cells infiltrating into the spinal cord.
H&E Staining of Brain Tissue from MBP-Induced EAE Model
Figure A. Low magnification and Figure B. High magnification of transverse sections of brain tissue demonstrating severe infiltration of inflammatory cells into the white matter.
H&E Staining of Transverse Section of Spinal Cord from MBP-Induced EAE Model
(A-D, Vehicle Group, Inflamed Spinal Cord Tissue): Figure A. Low magnification of inflammatory infiltrate in the white matter of the spinal cord. Figure B. Medium magnification of foci of inflammatory infiltrate. Figure C. High magnification shows mixed populations of mononuclear cells (black arrows). Figure D. Medium magnification of foci of inflammatory cells (black arrowheads).
E-G (Dexamethasone Group, normal appearance of the spinal cord): Figure E. Low magnification of the spinal cord showing minimal inflammatory infiltrate. Figure F. Medium magnification of a minimal foci of inflammatory infiltrate (circled). Figure G. High magnification of circled area in F showing several mononuclear cells (black arrows) surrounded by 2 blood vessels (asterisks) at the junction of the gray and white matter (black lines).
Other MS-EAE Models: