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Our 6-hydroxydopamine (6OHDA) model is a unilateral lesion model in which the Nigro-Striatal pathway is damaged. Since systemically administered 6OHDA fails to cross the blood-brain barrier, we instead stereotaxically inject toxin directly into the brain region of interest, typically the substantia nigra (SN), ventral tegmental area (VTA) or striatum.
Our scientists are experienced with rapidly validating models from literature, and can work with your study requirements. Contact a scientist to discuss your programs today and to learn how we can help accelerate your research.
Assessments:
IHC staining with Tyrosine Hydroxylase (TH) antibody. Staining of dopaminergic neurons, as TH is one of the enzymes involved in the production of Dopamine in neuronal cells.This staining is used in MPTP-induced PD model in mice or 6OHDA-induced PD model in rats.
Staining of 6-OHDA-induced Parkinson’s Disease Brain Sections in Rats
Figure A. Subgross image of Substantia Nigra sections showing reduction in number of TH-positive cells in the right hemisphere vs. the left intact hemisphere. Figure B. High magnification of TH-positive dopaminergic neurons. Figure C. Subgross image of TH-positive nerve fibers in section of striatum of the Left intact hemisphere contrasted to the lack of TH staining in the right hemisphere injected with 6-OHDA.
